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Inhibition of AR prevented inflammatory cytokines in the serum of STZ-induced diabetic mice. Mice were made diabetic as described in Methods. The diabetic mice were treated with fidarestat (10 mg/kg/d) for 1, 2, 3, and 4 days. At indicated time intervals, the levels of IL-1β, TNF-α, IL-1α, IFNγ, MIP-1α, MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using <t>Milliplex</t> <t>MAP</t> Kit mouse inflammatory <t>cytokine/chemokine</t> magnetic bead panel (Merck) along with Luminex xMAP detection method, per manufacturer’s protocol in a Millipore Multiplex system (Merck). ND, nondetectable levels. The bars represent the mean ± standard error of the mean (n ≥ 4). *P < 0.05 vs control; **P < 0.01 vs diabetic. Ctrl, control; Fid, fidarestat; ND, not detectable.
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Inhibition of AR prevented inflammatory cytokines in the serum of STZ-induced diabetic mice. Mice were made diabetic as described in Methods. The diabetic mice were treated with fidarestat (10 mg/kg/d) for 1, 2, 3, and 4 days. At indicated time intervals, the levels of IL-1β, TNF-α, IL-1α, IFNγ, MIP-1α, MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using <t>Milliplex</t> <t>MAP</t> Kit mouse inflammatory <t>cytokine/chemokine</t> magnetic bead panel (Merck) along with Luminex xMAP detection method, per manufacturer’s protocol in a Millipore Multiplex system (Merck). ND, nondetectable levels. The bars represent the mean ± standard error of the mean (n ≥ 4). *P < 0.05 vs control; **P < 0.01 vs diabetic. Ctrl, control; Fid, fidarestat; ND, not detectable.
Kit Milliplex Map Mouse High Sensitivity T Cell Magnetic Bead Panel Cat. Mhstcmag 70pk, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Inhibition of AR prevented inflammatory cytokines in the serum of STZ-induced diabetic mice. Mice were made diabetic as described in Methods. The diabetic mice were treated with fidarestat (10 mg/kg/d) for 1, 2, 3, and 4 days. At indicated time intervals, the levels of IL-1β, TNF-α, IL-1α, IFNγ, MIP-1α, MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using <t>Milliplex</t> <t>MAP</t> Kit mouse inflammatory <t>cytokine/chemokine</t> magnetic bead panel (Merck) along with Luminex xMAP detection method, per manufacturer’s protocol in a Millipore Multiplex system (Merck). ND, nondetectable levels. The bars represent the mean ± standard error of the mean (n ≥ 4). *P < 0.05 vs control; **P < 0.01 vs diabetic. Ctrl, control; Fid, fidarestat; ND, not detectable.
Milliplex Map Mouse Pituitary Magnetic Bead Panel Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA milliplex map human igf binding protein magnetic kit
Inhibition of AR prevented inflammatory cytokines in the serum of STZ-induced diabetic mice. Mice were made diabetic as described in Methods. The diabetic mice were treated with fidarestat (10 mg/kg/d) for 1, 2, 3, and 4 days. At indicated time intervals, the levels of IL-1β, TNF-α, IL-1α, IFNγ, MIP-1α, MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using <t>Milliplex</t> <t>MAP</t> Kit mouse inflammatory <t>cytokine/chemokine</t> magnetic bead panel (Merck) along with Luminex xMAP detection method, per manufacturer’s protocol in a Millipore Multiplex system (Merck). ND, nondetectable levels. The bars represent the mean ± standard error of the mean (n ≥ 4). *P < 0.05 vs control; **P < 0.01 vs diabetic. Ctrl, control; Fid, fidarestat; ND, not detectable.
Milliplex Map Human Igf Binding Protein Magnetic Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA human bone metabolism kit
Inhibition of AR prevented inflammatory cytokines in the serum of STZ-induced diabetic mice. Mice were made diabetic as described in Methods. The diabetic mice were treated with fidarestat (10 mg/kg/d) for 1, 2, 3, and 4 days. At indicated time intervals, the levels of IL-1β, TNF-α, IL-1α, IFNγ, MIP-1α, MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using <t>Milliplex</t> <t>MAP</t> Kit mouse inflammatory <t>cytokine/chemokine</t> magnetic bead panel (Merck) along with Luminex xMAP detection method, per manufacturer’s protocol in a Millipore Multiplex system (Merck). ND, nondetectable levels. The bars represent the mean ± standard error of the mean (n ≥ 4). *P < 0.05 vs control; **P < 0.01 vs diabetic. Ctrl, control; Fid, fidarestat; ND, not detectable.
Human Bone Metabolism Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA immunobeads milliplex map kit
Inhibition of AR prevented inflammatory cytokines in the serum of STZ-induced diabetic mice. Mice were made diabetic as described in Methods. The diabetic mice were treated with fidarestat (10 mg/kg/d) for 1, 2, 3, and 4 days. At indicated time intervals, the levels of IL-1β, TNF-α, IL-1α, IFNγ, MIP-1α, MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using <t>Milliplex</t> <t>MAP</t> Kit mouse inflammatory <t>cytokine/chemokine</t> magnetic bead panel (Merck) along with Luminex xMAP detection method, per manufacturer’s protocol in a Millipore Multiplex system (Merck). ND, nondetectable levels. The bars represent the mean ± standard error of the mean (n ≥ 4). *P < 0.05 vs control; **P < 0.01 vs diabetic. Ctrl, control; Fid, fidarestat; ND, not detectable.
Immunobeads Milliplex Map Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA milliplex® map human high sensitivity t cell magnetic bead panel 96-well plate assay
Inhibition of AR prevented inflammatory cytokines in the serum of STZ-induced diabetic mice. Mice were made diabetic as described in Methods. The diabetic mice were treated with fidarestat (10 mg/kg/d) for 1, 2, 3, and 4 days. At indicated time intervals, the levels of IL-1β, TNF-α, IL-1α, IFNγ, MIP-1α, MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using <t>Milliplex</t> <t>MAP</t> Kit mouse inflammatory <t>cytokine/chemokine</t> magnetic bead panel (Merck) along with Luminex xMAP detection method, per manufacturer’s protocol in a Millipore Multiplex system (Merck). ND, nondetectable levels. The bars represent the mean ± standard error of the mean (n ≥ 4). *P < 0.05 vs control; **P < 0.01 vs diabetic. Ctrl, control; Fid, fidarestat; ND, not detectable.
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Inhibition of AR prevented inflammatory cytokines in the serum of STZ-induced diabetic mice. Mice were made diabetic as described in Methods. The diabetic mice were treated with fidarestat (10 mg/kg/d) for 1, 2, 3, and 4 days. At indicated time intervals, the levels of IL-1β, TNF-α, IL-1α, IFNγ, MIP-1α, MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using Milliplex MAP Kit mouse inflammatory cytokine/chemokine magnetic bead panel (Merck) along with Luminex xMAP detection method, per manufacturer’s protocol in a Millipore Multiplex system (Merck). ND, nondetectable levels. The bars represent the mean ± standard error of the mean (n ≥ 4). *P < 0.05 vs control; **P < 0.01 vs diabetic. Ctrl, control; Fid, fidarestat; ND, not detectable.

Journal: Endocrinology

Article Title: Aldose Reductase Mediates NLRP3 Inflammasome–Initiated Innate Immune Response in Hyperglycemia-Induced Thp1 Monocytes and Male Mice

doi: 10.1210/en.2017-00294

Figure Lengend Snippet: Inhibition of AR prevented inflammatory cytokines in the serum of STZ-induced diabetic mice. Mice were made diabetic as described in Methods. The diabetic mice were treated with fidarestat (10 mg/kg/d) for 1, 2, 3, and 4 days. At indicated time intervals, the levels of IL-1β, TNF-α, IL-1α, IFNγ, MIP-1α, MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using Milliplex MAP Kit mouse inflammatory cytokine/chemokine magnetic bead panel (Merck) along with Luminex xMAP detection method, per manufacturer’s protocol in a Millipore Multiplex system (Merck). ND, nondetectable levels. The bars represent the mean ± standard error of the mean (n ≥ 4). *P < 0.05 vs control; **P < 0.01 vs diabetic. Ctrl, control; Fid, fidarestat; ND, not detectable.

Article Snippet: At indicated time intervals, the levels of IL-1 β , TNF- α , IL-1 α , IFN γ , MIP-1 α , MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using Milliplex MAP Kit mouse inflammatory cytokine/chemokine magnetic bead panel (Merck) along with Luminex xMAP detection method, per manufacturer’s protocol in a Millipore Multiplex system (Merck).

Techniques: Inhibition, Luminex, Multiplex Assay